A new molecular research tool to detect H. pylori in saliva.
Finally, scientists have devised a new approach using DNA sequencing to detect H. pylori in saliva and to identify the type of strain causing the infection. The method has the potential to be a much cheaper and faster method of screening for the bacterium.
This approach also helps to identify the subtype of the pathogenic H. pylori strain and to map the transmission route of the disease, which could be useful for disease control.
The method involves targeting the 16S rRNA gene and this has been done using the primers Hpy-1 and Hpy-2.
Both of these primers target a region of the H. pylori 16S rRNA gene that has been conserved over a long time, and this is why they can detect H. pylori in both saliva and gastric biopsy specimens.
doi:10.1289/jlb.1408-0166.2010.71561.x K. KAWASAKI,A. KAYASHI,M. SADA,K. KAWAMOTO, H. IMAI,K. KOBAYASHI, M. MORI.
H. pylori infection
has become the most common chronic disease in Japan in the 1990s.
According to the Ministry of Health, Labor, and Welfare, Japan, the number of newly diagnosed cases of H. pylori infection increased from 60,000 in 1996 to 120,000 in 2001, which was almost double the number in 1990.
The total number of reported H. pylori-associated diseases is enormous.
In Japan, stomach cancer is ranked first among cancers in adults.
According to the Cancer Research Institute of the Japanese Foundation for Cancer Research, it is estimated that about 30,000 stomach cancers will be diagnosed in 2010 and that deaths from stomach cancer will exceed those from breast cancer.
Epidemiological studies conducted in Japan have shown that age-specific incidences of gastric cancer increase sharply after the fifth decade of life,
ranging from less than 3% in those aged 20 to 29 years to 90% in those aged 80 years or older.
The overall incidence of stomach cancer in Japan is the highest in the world, but the mortality rate is relatively low.
It is believed that this may be due to early detection, by the mass screening of H. pylori infection.
Early detection of 01e38acffe
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